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25-OH Vitamin D2/D3 from Plasma using Strata DE SLE and a Kinetex core-shell EVO C18 LC Column

Application Detail (App ID: 24552)

Part No:00B-4725-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% formic acid in water
Elution B:0.1% formic acid in acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 40 60
2 0.83 5 95
3 3.33 5 95
4 3.34 40 60
5 5.83 40 60

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™

Analyte Details

Analyte Detail not available

1
25-OH Vitamin D3
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2
25-OH Vitamin D2
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