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Applications
Chloramphenicol - Alternative Method
3312
Separation Mode: Reversed Phase
Reversed Phase
USP L01
C18(2)
HPLC
Anti-Bacterial Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Chloramphenicol - Alternative Method
Analytes
1Chloramphenicol
Compound Name
Chloramphenicol
CID
5959
Molecular Formula
C11H12Cl2N2O5
Molecular Weight
323
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
Official Method: USP L01
LC Conditions (App ID: 3312)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: Water with 0.05% Formic Acid
B: Methanol with 0.05% Formic Acid
Gradient Profile:
Step No.Time(min)%A%B
107030
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 280 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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