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Applications
Phenols on Envirosep-PP (EPA Method 604)
5304
Separation Mode: Reversed Phase
Reversed Phase
EPA 604
PP
HPLC
Uncoupling Agents
Environmental
Pollutant / Contaminant
Environmental / Health

Applications

Phenols on Envirosep-PP (EPA Method 604)
Analytes
1Phenol
24-Nitrophenol
32,4-Dinitrophenol
42-Nitrophenol
5o-Chlorophenol
64,6-Dinitrophenol
72,4-Dimethylphenol
84-Chloro-3-methylphenol
92,4-Dichlorophenol
102,4,6-Trichlorophenol
11Pentachlorophenol
Compound Name
Phenol
CID
996
Molecular Formula
C6H6O
Molecular Weight
94
No. Hydrogen Bond Acceptors
1
No. Hydrogen Bond Donors
1
Details
Official Method: EPA 604
LC Conditions (App ID: 5304)
Column:
Brand Name:
Part No:
Phase Name:
PP
Elution Type:
Gradient
Mobile Phase:
A: Water
B: Methanol both with 1% acetic acid
Gradient Profile:
Step No.Time(min)%A%B
107030
29.54456
3124456
4130100
Flow Rate:
0.5 mL/min
Temperature:
45°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 280 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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