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Applications
Glucocorticoids - Sphereclone vs. Spherisorb Comparison
5546
Separation Mode: Reversed Phase
Reversed Phase
ODS(2)
HPLC
Anti-Inflammatory Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Glucocorticoids - Sphereclone vs. Spherisorb Comparison
Analytes
1Uracil
2Prednisolone
3Betamethasone
4Prednisolone 21-acetate
5Betamethasone 17-valerate
Compound Name
Uracil
CID
1174
Molecular Formula
C4H4N2O2
Molecular Weight
112
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 5546)
Column:
Brand Name:
Part No:
Phase Name:
ODS(2)
Elution Type:
Isocratic
Mobile Phase:
A: Acetonitrile
B: Water
Gradient Profile:
Step No.Time(min)%A%B
105050
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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