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Frequently Asked Questions (FAQs) for Small Molecule Analysis


Advancing Small Molecule HPLC Analysis

Most pharmaceutical drugs on the market are in the form of small molecules. Small molecules and their metabolites are commonly studied by HPLC chromatography where they are analyzed, separated, and purified with HPLC columns ».

HPLC analysis of small molecules mostly involves reversed phase HPLC chromatography but can also engage normal phase HPLC chromatography and ion-exchange HPLC chromatography. HPLC columns typically contain spherical silica beads of a particular particle size and bonded stationary phase. The evolution of HPLC chromatography shows a trend for HPLC columns to possess smaller particle sizes, offer high efficiency and lower instrument backpressure.

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Method Development and Product Selection

Selectivity Tips for Kinetex Core-shell and Luna Omega LC columns »

A quick and easy reference that outlines when core-shell technology columns and thermally modified fully porous particle columns should be used.

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Frequently Asked Questions (FAQs) for Biomolecule Analysis


Quickly Analyze and Separate Biomolecules

In the biopharmaceutical industry, large molecules that interact with a biological system are all known as biomolecules. Examples of biomolecules include amino acids, peptides, proteins, antibodies, and DNA/RNA oligonucleotides.

Phenomenex offers a variety of specialized HPLC columns for the analysis and separation of biomolecules. In the case of protein analysis, our columns allow scientists to determine protein concentration, as well as the ability to scale up to protein purification. The columns designed for protein analysis contain silica particles with larger pore sizes so they can better interact with the biomolecules injected onto the column. It’s these interactions that separate the proteins to determine protein concentration and eventually scale the process up to protein purification.

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Method Development and Product Selection

BioSeparations Handbook »

Practical method development strategies to the challenges of characterization, isolation, and purification of biomolecules.

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Frequently Asked Questions (FAQ) for Chiral Analysis


Achieve High Purity Chiral HPLC Separations

Effective chiral separations have become increasingly important to the pharmaceutical and biopharmaceutical industries. The rapid introduction of optically active medicinal drugs, along with increasing government regulation, necessitates that rapid, sensitive, and reliable stereochemical methods be devised for their analysis. Chiral chromatography is by far the most powerful and sensitive analytical technique for resolving enantiomers.

Phenomenex maintains a dedicated chiral screening services laboratory using our own chiral HPLC columns which can assist you in developing or improving your chiral separation. We can assist you in column selection, method development, scale up, or any other aspect of chiral chromatography.

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  • Lux Chiral LC Columns »
    Polysaccharide based chiral columns for effective screening under Reversed Phase, Polar Organic, Normal Phase, and SFC conditions.
 

Method Development and Product Selection

Chiral Selection Guide »

Learn three easy ways to to simplify your chiral HPLC method development.

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Frequently Asked Questions (FAQ) for Excipient Analysis


Reliable Excipient Analysis

Excipients are the inactive ingredients within a pharmaceutical formulation that hold the active pharmaceutical ingredient (API) and ensure the conveniently sized dose is delivered efficiently and effectively to the targeted area. Excipients are typically inert substances, but sometimes they can give adverse side effects. Modern pharmaceutical regulations require formulation analysis for all ingredients within a pharmaceutical drug to identify all excipient components and guarantee safety of the end product.

Formulation analysis can be achieved through several modes of chromatography including reversed phase, ion-exclusion, and size exclusion chromatography (SEC), which includes gel permeation chromatography (GPC) and gel filtration chromatography (GFC). We offer a wide breadth of products for reliable and reproducible excipient analysis.

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Method Development and Product Selection

Formulations Guide »

A complete guide to formulations analysis including Ph. Eur and USP recommendations

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Frequently Asked Questions (FAQ) for Residual Solvent Analysis


Reliable Residual Solvent Testing and Confirmation

The USP has provided a method for the identification, control, and quantification of organic volatile impurities (OVIs) and Class 1 and 2 residual solvents, which specifies analytical procedures by GC/FID. Phenomenex offers G16 and G43 phases used in USP Monograph <467>, as well as a collection of tips, tools, and resources to improve and optimize residual solvent testing.

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Method Development and Product Selection