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UHPLC: Small Particle Solutions for Big Chromatography Challenges »
LC:Transforming Small Molecule Method Development with Core-shell Technology »
Most pharmaceutical drugs on the market are in the form of small molecules. Small molecules and their metabolites are commonly studied by HPLC chromatography where they are analyzed, separated, and purified with HPLC columns ».
HPLC analysis of small molecules mostly involves reversed phase HPLC chromatography but can also engage normal phase HPLC chromatography and ion-exchange HPLC chromatography. HPLC columns typically contain spherical silica beads of a particular particle size and bonded stationary phase. The evolution of HPLC chromatography shows a trend for HPLC columns to possess smaller particle sizes, offer high efficiency and lower instrument backpressure.
Find the latest webinars, technical notes, and guides for semi-volatile analysis..
Reduce run times, achieve higher resolution, and stay within Allowable Adjustments with this easy to navigate guide.
Article to help understand the latest revision of USP Chapter <621> that further clarifies what “allowable adjustments” can be made to methods without having to revalidate.
Go beyond what is traditionally accepted for UHPLC runs with ultra-high efficiency and low backpressures.
A look at core-shell technology and its impact on pharmacuetical HPLC.
Do I Really Know How Much Aggregate is in my Protein Therapeutic? »
Method Development Strategies for Intact Protein Analysis by Reversed Phase and Gel Filtration Chromatography »
In the biopharmaceutical industry, large molecules that interact with a biological system are all known as biomolecules. Examples of biomolecules include amino acids, peptides, proteins, antibodies, and DNA/RNA oligonucleotides.
Phenomenex offers a variety of specialized HPLC columns for the analysis and separation of biomolecules. In the case of protein analysis, our columns allow scientists to determine protein concentration, as well as the ability to scale up to protein purification. The columns designed for protein analysis contain silica particles with larger pore sizes so they can better interact with the biomolecules injected onto the column. It’s these interactions that separate the proteins to determine protein concentration and eventually scale the process up to protein purification.
Practical method development strategies to the challenges of characterization, isolation, and purification of biomolecules.
A look at how core-shell technology can dramatically improve analysis of biomolecules
Achieve high resolution GFC with Yarra SEC columns
With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation.
Improved resolution of proteins is accomplished with a new core-shell particle morphology which minimizes protein band broadening that occurs during diffusion in and out of the core-shell particle.
Chiral HPLC Introduction: Method Development and Applications »
Effective chiral separations have become increasingly important to the pharmaceutical and biopharmaceutical industries. The rapid introduction of optically active medicinal drugs, along with increasing government regulation, necessitates that rapid, sensitive, and reliable stereochemical methods be devised for their analysis. Chiral chromatography is by far the most powerful and sensitive analytical technique for resolving enantiomers.
Phenomenex maintains a dedicated chiral screening services laboratory using our own chiral HPLC columns which can assist you in developing or improving your chiral separation. We can assist you in column selection, method development, scale up, or any other aspect of chiral chromatography.
Learn three easy ways to to simplify your chiral HPLC method development.
Lux Replaces DAICEL CHIRALCEL and CHIRALPAK Columns at Substantial Cost Savings!
Five different Lux polysaccharide-based chiral stationary phases were explored, demonstrating the feasibility of LC/MS/MS analysis of acidic pharmaceutical racemates.
Comparison of two chiral stationary phases (Lux Cellulose-1 and CHIRALPAK®IB) with the chiral selector cellulose tris(3,5-dimethyl- phenylcarbamate)
Access our simplified chiral HPLC/SFC column screening strategies.
Basic HPLC Troubleshooting
and Column Care »
Excipients are the inactive ingredients within a pharmaceutical formulation that hold the active pharmaceutical ingredient (API) and ensure the conveniently sized dose is delivered efficiently and effectively to the targeted area. Excipients are typically inert substances, but sometimes they can give adverse side effects. Modern pharmaceutical regulations require formulation analysis for all ingredients within a pharmaceutical drug to identify all excipient components and guarantee safety of the end product.
Formulation analysis can be achieved through several modes of chromatography including reversed phase, ion-exclusion, and size exclusion chromatography (SEC), which includes gel permeation chromatography (GPC) and gel filtration chromatography (GFC). We offer a wide breadth of products for reliable and reproducible excipient analysis.
A complete guide to formulations analysis including Ph. Eur and USP recommendations
Tween 80 is used as an emulsifier for dissolving oils, as well as a surfactant. In this application, Tween 80 is analyzed by HPLC-UV using a Yarra 1.8 μm SEC-X150 150 x 4.6 mm aqueous size exclusion (SEC), or gel filtration chromatography (GFC), column in under 12 minutes.
Analyzing Pharmaceutical Tablet and Capsule Excipients by Gel Permeation Chromatography (GPC) using Phenogel Columns.
Rezex RPM, RCM and RCU phases are well-suited for use in USP methodologies for the analysis of mannitol, sorbitol, xylitol, and other excipients.
A look at the impact of the revised USP Chapter <467>
Critical Choices: Best Practices for Improving GC Methods »
GC Troubleshooting Tips & Tricks From Injection Through Detection »
The USP has provided a method for the identification, control, and quantification of organic volatile impurities (OVIs) and Class 1 and 2 residual solvents, which specifies analytical procedures by GC/FID. Phenomenex offers G16 and G43 phases used in USP Monograph <467>, as well as a collection of tips, tools, and resources to improve and optimize residual solvent testing.
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