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One of the World's

Leading HPLC Columns

Luna HPLC Information


One of the World's Leading HPLC Columns

Dependable, ultra-pure silica-based HPLC columns that offer an extensive variety of selectivities which are scalable from micro-bore to preparative and purification scale solutions.

  • Extensive line of rugged USP phases
  • Wide pH stability for long column lifetime and method flexibility
  • Easy method scalability from Fast LC to Preparative and Bulk Purification

C18(2) Phase Detail


Particle Sizes (µm)2.5, 3, 5, 10, 15
Pore Size (Å)100
Surface Area (sq. m/g)400
Carbon Load (%)17.5
pH Range1.5-10.0
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C18(2) Phase Information


Octadecyl silane ligands are bound to the silica surface, making for a very hydrophobic phase with great methylene selectivity. Non-polar endcapping virtually eliminates silanol interactions

PhaseParticle Sizes (µm)Pore Size (Å)Surface Area (sq. m/g)Carbon Load (%)pH RangeRecommended Use
C183, 5, 10100440191.5-10.0Separation of hydrophobic compounds
C18(2)2.5, 3, 5, 10, 1510040017.51.5-10.0Separation of hydrophobic compounds
C55, 1010044012.51.5-10.0Separation of extremely hydrophobic compounds
C83, 5, 1010044014.751.5-10.0Separation of very hydrophobic compounds
C8(2)3, 5, 10, 1510040013.51.5-10.0Separation of very hydrophobic compounds
CN3, 5, 1010040071.5-7.0Separation of polar compounds
HILIC3, 5200200*5.72.0-7.5Separation of polar compounds
NH23, 5, 101004009.51.5-11.0Separation of polar compounds
PFP(2)3, 510040011.51.5-8.0Separation of aromatic and halogenated compounds
Phenyl-Hexyl3, 5, 10, 1510040017.51.5-10.0Separation of aromatic compounds
PREP C18(2)10100N/A 
PREP C18(3)10100N/A 
PREP C410100N/A 
PREP C8(2)10100N/A 
PREP Phenyl-Hexyl10100N/A 
PREP Silica(2)10100N/A 
PREP Silica(3)10100N/A 
SCX5, 101004000.55 % Sulfur Load2.0-7.0Separation of positively charged compounds
Silica (2)3, 5, 10, 15100400N/A2.0-7.5Separation of polar compounds
Document Type:
Document Type Title
Brochures Axia Preparative HPLC/ SFC Brochure Add to My Documents
Brochures Bulk Media Selection Guide Add to My Documents
Other Clinical Research Solutions Guide Add to My Documents
Technical Notes Optimization Tips for HILIC Analysis of Basic Compounds (TN-1040) Add to My Documents
Brochures Explore Luna HPLC Columns Add to My Documents
Brochures Extend your Analytical and Preparative HPLC/ SFC Column Lifetimes Add to My Documents
Technical Notes Fast and Simple Analysis of Polyphenols in Red Wine (TN-1166) Add to My Documents
Technical Notes Increasing LC-MS MS Sensitivity with Luna HILIC Add to My Documents
Brochures Luna HPLC Columns Add to My Documents
Brochures Media Selection Guide Add to My Documents
Other A Simple, Rapid Method for teh Analysis of Ethephon from Ketchup using Solid Phase Extraction and LCMSMS Add to My Documents
Technical Notes Achiral Stationary Phase Selectivity under SFC Conditions (TN-1041) Add to My Documents
Technical Notes Glucosinolates from Cruciferous Vegetable Extracts by HPLC/UV (TN-1157) Add to My Documents
Supporting Docs Guidelines for HILIC Method Development Add to My Documents
Supporting Docs Luna 2.5 µm C18(2) HST White Paper Add to My Documents
Technical Notes Metanephrine and Normetanephrine from Plasma by LC/MS/MS (TN-0018) Add to My Documents
Technical Notes Peptide Separations by Cation Exchange HPLC (TN-1024) Add to My Documents
Brochures Preparative Columns Redefined Add to My Documents
Technical Notes Thiamin Diphosphate (Vitamin B1) from Whole Blood by LC-Fluorescence Add to My Documents
Brochures Luna C18 (3) Add to My Documents
Technical Notes Optimization of Preparative Production using Axia™ for Increased Purity, Yield and Productivity (TN-1049) Add to My Documents
Technical Notes Peptide Purification Method Development: Application for the Purification of Bivalirudin (TN-1129) Add to My Documents
Technical Notes Melamine from Food Products by SPE and LC/MS/MS (TN-0019) Add to My Documents
Technical Notes Free Metanephrine (MN) and Normetanephrine (NMN) from Plasma by SPE and LC/MS/MS (TN-0018) Add to My Documents
Brochures Preparative/Process Add to My Documents
Brochures SecurityGuard Column Protection Family Brochure (UHPLC, HPLC, PREP, SFC) Add to My Documents
Supporting Docs Axia Packed Columns Vs Traditional Packed Preparative Columns (Video) Add to My Documents
Supporting Docs HPLC Column Care Guide Add to My Documents
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