25-OH Vitamin D2 and D3 from Serum by LC/MS/MS (TN-1055)

This technical note describes a sensitive, rugged and robust, highthroughput method for the analysis of 25-OH vitamin D2 and 25-OH vitamin D3 in serum. Following protein precipitation to eliminate proteins and disrupt serum protein binding of 25-OH vitamin D2 and 25-OH vitamin D3, the chromatographic separation of 25-OH vitamin D2 and 25-OH vitamin D3 is carried out in less than 5 minutes using a Kinetex® 2.6 μm C18 core-shell column, with total chromatographic cycle time of 6 minutes. This method provides rapid, sensitive, rugged and robust LC/MS/MS analysis of Vitamin D levels in patient serum (LOD of 1 and 2 ng/mL for 25-OH D3 and 25-OH D2, respectively with CV of 4-7 %) and speeds the diagnosis by hospital and clinical laboratories of potential vitamin D deficiencies.
Document Type:
Technical Notes
HPLC / UHPLC Sample Prep
Kinetex Impact
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