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25-OH Vitamin D2 and D3 from Serum by LC/MS/MS (TN-1055)


Abstract:
This technical note describes a sensitive, rugged and robust, highthroughput method for the analysis of 25-OH vitamin D2 and 25-OH vitamin D3 in serum. Following protein precipitation to eliminate proteins and disrupt serum protein binding of 25-OH vitamin D2 and 25-OH vitamin D3, the chromatographic separation of 25-OH vitamin D2 and 25-OH vitamin D3 is carried out in less than 5 minutes using a Kinetex® 2.6 μm C18 core-shell column
Document Type:
Technical Notes
Industries:
Pharmaceutical/Biopharmaceutical
Techniques:
HPLC / UHPLC
Brands:
Kinetex
PDF Link:
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