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Applications
Extraction of Diazepam on Strata™ X
14618
Separation Mode: Reversed Phase
Reversed Phase
Polar-RP
HPLC
Antiemetics
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

Extraction of Diazepam on Strata™ X
Analytes
1Desmethyldiazepam
2Diazepam
Compound Name
Nordiazepam
CID
2997
Molecular Formula
C15H11ClN2O
Molecular Weight
270
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 14618)
Column:
Brand Name:
Part No:
Phase Name:
Polar-RP
Elution Type:
Gradient
Mobile Phase:
A: 20 mM KH2PO4 (pH=2.5)
B: MeOH
Gradient Profile:
Step No.Time(min)%A%B
107525
237525
383070
4113070
5147525
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, POLAR-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-6076 - Holder Part No.: KJ0-4282

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