Applications

Extraction of Barbiturates from Urine on Strata™ X

Analytes

1Phenobarbital
2Butabarbital
3Butalbital
4Amobarbital
5Secobarbital

Compound Name Phenobarbital
CID 4763
Molecular Formula C12H12N2O3
Molecular Weight 232
No. Hydrogen Bond Acceptors 3
No. Hydrogen Bond Donors 2

Details

LC Conditions (App ID: 14622)

Column:

Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Synergi

Part No:

00F-4337-E0

Phase Name:

Max-RP

Elution Type:

Gradient

Mobile Phase:

A: 20 mM KH2PO4, pH 7.0

B: Acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	80	20
2	3	80	20
3	15	50	50
4	16	80	20

Flow Rate:

1 mL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Fixed Wave. (UV) @ 210 nm (22°C)

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00F-4337-E0

Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Qty:

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