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Applications
Tricyclic Antidepressants at Neutral pH by Gemini 5um C18
15595
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antidepressive Agents, Tricyclic
Pharmaceutical
Therapeutic / Clinical

Applications

Tricyclic Antidepressants at Neutral pH by Gemini 5um C18
Analytes
1Nortriptyline
2Imipramine
3Amitriptyline
4Clomipramine
Compound Name
Nortriptyline
CID
4543
Molecular Formula
C19H21N
Molecular Weight
263
No. Hydrogen Bond Acceptors
1
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 15595)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Isocratic
Mobile Phase:
A: 20mM Phosphate buffer pH7.0
B: Acetonitrile
C: Methanol
Gradient Profile:
Step No.Time(min)%A%B%C
10303535
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
Peak 3 -- Amitriptyline Efficiency = 9183 Peak Symmetry = 0.80

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