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Applications

Jupiter® 300 C4 - Effect of Temperature on PEGylated Protein Separations

Analytes

1PEGylated Carbonic anhydrase

Analyte not found

Details

LC Conditions (App ID: 16196)

Column:

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Part No:

Phase Name:

C4

Elution Type:

Gradient

Mobile Phase:

A: 0.1% TFA in Water

B: 90% ACN 0.09% TFA in Water

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	80	20
2	25	45	55

Flow Rate:

1 mL/min

Temperature:

45°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)

Sample Note:

Reversed phase runs of PEGylated carbonic anhydrase at different temperatures. Increasing the temperature to 45°C slightly improves resolution of different PEG forms.

Order Items Used in This Application

00F-4167-E0

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Qty:

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