| 1PEGylated Ribonuclease A |
|---|
Analyte not found
Applications
Jupiter® 300 C4 - Effect of Varying Mobile Phase Gradient Conditions
16197
Separation Mode: Reversed Phase
Reversed Phase
C4
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Biochemical - Polymeric
Biochemical Compound / Nutrient
Analytes
Details
LC Conditions (App ID: 16197)
Column: | ||||||||||||
Brand Name: | ||||||||||||
Part No: | ||||||||||||
Phase Name: C4 | ||||||||||||
Elution Type: Gradient | ||||||||||||
Mobile Phase: A: 0.1% TFA in Water B: 90% ACN 0.09% TFA in Water | ||||||||||||
Gradient Profile:
| ||||||||||||
Flow Rate: 1 mL/min | ||||||||||||
Temperature: 45°C | ||||||||||||
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C) | ||||||||||||
Sample Note: Reversed phase runs of PEGylated ribonuclease A using different gradient conditions on a Jupiter 300 C4 column (gradient length 25 min). Note that if initial %B is increased much above 25% then retention and resolution of PEGylated forms is lost. Gradient slopes of between 1-2%/min appear to be a good compromise between resolution and run time. |
Order Items Used in This Application
00F-4167-E0
Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea
Qty:
Similar Applications
search
PEGylated Ribonuclease A
BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea
Gel Filtration Chromatography (GFC)
Jupiter 3 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea
Gel Filtration Chromatography (GFC)
Jupiter 3 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
CONNECT WITH US
