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Applications
Biogenic Amines in Fish and Fish Products According to § 35 LMBG
17911
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Histamine Agents
Biochemical - NOT Polymeric (incl. oligomers)
Biochemical Compound / Nutrient

Applications

Biogenic Amines in Fish and Fish Products According to § 35 LMBG
Analytes
1Tyramine
2Putrescine
3Cadaverine
4Histamine
5Agamatine
6Spermidine
7Spermine
Compound Name
Tyramine
CID
5610
Molecular Formula
C8H11NO
Molecular Weight
137
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 17911)
Column:
Brand Name:
Part No:
Phase Name:
NX-C18
Elution Type:
Gradient
Mobile Phase:
A: Water + 100 mM NaOAc, pH 4.5 + 10 mM Sodium 1-octanesulfonate
B: Water + 155 mM NaOAc, pH 4.5 + 10 mM Sodium 1-octanesulfonate / ACN (77:23)
Gradient Profile:
Step No.Time(min)%A%B
108020
2206040
3282575
4342575
534.10100
6500100
750.18020
8608020
Flow Rate:
1 mL/min
Temperature:
20°C
Detection:
Fluorescence (FLUOR) @ 330 nm (20°C)
Detector Info:
Online post-column derivatization with OPA
Sample Note:
The application was kindly provided by the Amt für Verbraucherschutz in Mettmann, Germany.

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