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Applications
Column Diameter Study with Luna 5u C18(2) 50 x 21.2mm
18063
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Antipruritics
Pharmaceutical
Therapeutic / Clinical

Applications

Column Diameter Study with Luna 5u C18(2) 50 x 21.2mm
Analytes
1Propranolol
2Diphenhydramine
Compound Name
Inderal
CID
62882
Molecular Formula
C16H22ClNO2
Molecular Weight
295
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 18063)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Gradient
Mobile Phase:
A: 0.5% TFA in Acetonitrile
B: 0.5% TFA in Water
Gradient Profile:
Step No.Time(min)%A%B
10595
25955
Flow Rate:
30
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
Comparision of column diameter on preparative separations using Axia-packed columns in various diameters: 4.6 (conventional hardware), 21.2, 30 and 50mm (cf: App IDs: 18062 - 18065). Load varied by diameter as follows: - 4.6mm: 64 µg in 21 µL DMSO (scaling factor = 1) - 21.2mm: 1.2 mg in 420 µL DMSO (scaling factor = 20) - 30mm: 2.4 mg in 840 µL DMSO (scaling factor = 40) - 50mm: 7.3 mg in 2100 µL DMSO (scaling factor = 118)

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