| 1Melamine |
|---|
| 2Melamine 13C3-Amino 15N3 |
Compound Name Melamine |
CID 7955 |
Molecular Formula C3H6N6 |
Molecular Weight 126 |
No. Hydrogen Bond Acceptors 6 |
No. Hydrogen Bond Donors 3 |
Applications
Calibration Curve for Melamine Extraction (20-2000ng/mL range) from Strata Melamine on Luna HILIC
18467
Pollutant / Contaminant
Environmental / Health
Applications
Calibration Curve for Melamine Extraction (20-2000ng/mL range) from Strata Melamine on Luna HILIC
Analytes
Details
LC Conditions (App ID: 18467)
Elution Type: Isocratic | ||||||
Mobile Phase: A: 90/10 ACN/100 mM Ammonium Formate, pH 3.2 | ||||||
Gradient Profile:
| ||||||
Flow Rate: 0.4 mL/min | ||||||
Temperature: 22°C | ||||||
Detection: Mass Spectrometer (MS) (22°C) | ||||||
Detector Info: * MS in -ve ionization till 1.7 mins then to +ve till 3.5 mins | ||||||
Sample Note: LC/MS/MS Condition:
Column: Luna 3µm HILIC, 100x2.0mm; Flow rate: 0.4ml/min
Mobile Phase: Isocratic; 90/10 ACN/100mM NH4 Formate pH 3.2; MS Detector: API 3000 LC/MS/MS with ESI (TurboIonSpray®) (www.Appliedbiosystems.com), Switch from -ve Ion Mode (for cyanuric) to Positive Ion (for Melamine);
MRM; heater gas flow 7000 cc/min; heater temperature 450 °C
Melamine QC - 200 ng
% Accuracy 99.45%
% CV 6%
Calibration Curve of Extracted Samples
conc. (ng) % Accuracy
20 102%
40 96%
200 104%
400 98%
2000 100.03% |
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