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Applications
Methadone on Kinetex 2.6µm C18 50 x 2.1mm ID with Acquity UPLC MS/MS
18609
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

Methadone on Kinetex 2.6µm C18 50 x 2.1mm ID with Acquity UPLC MS/MS
Analytes
1Methadone
2Methadone-d9
Compound Name
Methadone
CID
4095
Molecular Formula
C21H27NO
Molecular Weight
309
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 18609)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
109010
213565
31.01595
41.5595
51.519010
639010
Flow Rate:
700 µL/min
Temperature:
0°C
Detection:
Mass Spectrometer (MS) (110°C)
Detector Info:
ESI+, MRM mode Methadone - 310.2-265.2 (quantitation) & 310.2-105.1 (qualifier) d9-methadone - 319.2-268.2 (internal standard)

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