| 1IgG |
|---|
| 2IgG2 trimer |
| 3IgG dimer |
| 4IgG dimer |
| 5IgG monomer |
| 6IgG |
Analyte not found
Applications
Hu IgG2k aggregates on BioSep3000
18906
Separation Mode: Gel Filtration Chromatography (GFC)
Gel Filtration Chromatography (GFC)
SEC-s3000
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Protein
Biochemical Compound / Nutrient
Analytes
Details
LC Conditions (App ID: 18906)
Column: | ||||||
Brand Name: | ||||||
Part No: | ||||||
Phase Name: SEC-s3000 | ||||||
Elution Type: Isocratic | ||||||
Mobile Phase: A: 100mM Sodium Phosphate pH 6.8 | ||||||
Gradient Profile:
| ||||||
Flow Rate: 1 mL/min | ||||||
Temperature: 22°C | ||||||
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 220 nm (22°C) | ||||||
Sample Note: Application Focus: An example of Ig-G aggregate testing on BioSep 3000
Analyzing Ig-G based therapeutics for aggregates is probably the most common application that is performed on a GFC column. Most users are attempting to get best resolution possible between the Ig-G monomer peak and any associated dimers in the sample. In this example human Ig-G2 is injected on a BioSep 3000 column (300x7.8 mm) and dimer and aggregate is quantitated.
Results show that the dimer peak of Ig-G is well resolved from the monomer peak, and that there appears to be two different dimer forms that are partially resolved by the BioSep 3000 column. In addition, there appears to be aggregate at the total excluded void of the column and the appearance of a possible trimer peak ahead of the dimer peak. Finally, there is a fragment peak that elutes after the Ig-G monomer peak, most likely attributed to an Ig-G that is missing one of its Fab fragment. These results show the utility of using BioSep 3000 for antibody aggregate analysis. |
Order Items Used in This Application
00H-2146-K0
BioSep 5 µm SEC-s3000 290Å, LC Column 300 x 7.8 mm, Ea
Qty:
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