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Applications
Hydrochlorothiazide Related Substance Analysis on Luna 5u C18 250 x 4.6mm ID
18932
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Antihypertensive Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Hydrochlorothiazide Related Substance Analysis on Luna 5u C18 250 x 4.6mm ID
Analytes
1Chlorothiazide
2Hydrochlorothiazide
Compound Name
Chlorothiazide
CID
2720
Molecular Formula
C7H6ClN3O4S2
Molecular Weight
295
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 18932)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: 100mM Na Phosphate Buffer pH=3.0
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Detector Info:
MBP=190 Bar
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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