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Applications
25-OH Vitamin D2 and D3 Standards from Serum on Fully Porous 5µm C18 after PPT
19315
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Vitamins
Clinical
Therapeutic / Clinical
Therapeutic / Clinical

Applications

25-OH Vitamin D2 and D3 Standards from Serum on Fully Porous 5µm C18 after PPT
Analytes
125-Hydroxy vitamin D2 (25-OH D2)
225-Hydroxy vitamin D3-d6 (25-OH D3-d6)
325-Hydroxy vitamin D2 (25-OH D2)
Compound Name
25-Hydroxycalciferol
CID
5710148
Molecular Formula
C28H44O2
Molecular Weight
412
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 19315)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Gradient
Mobile Phase:
A: 0.05 % Formic acid
B: 5 mM Ammonium acetate + 0.1 % Formic acid in methanol
Gradient Profile:
Step No.Time(min)%A%B
10928
25928
32050100
42900100
5360928
Flow Rate:
0.9 mL/min
Temperature:
35°C
Detection:
Mass Spectrometer (MS) (22°C)
Detector Info:
SCIEX API 4000™
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-4286 - Holder Part No.: KJ0-4282

Order Items Used in This Application

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