| 1Digoxin |
|---|
| 2Oleandrin |
| 3Digitoxin |
Compound Name Digoxin |
CID 30322 |
Molecular Formula C41H64O14 |
Molecular Weight 780 |
No. Chiral Centers: 1 |
No. Hydrogen Bond Acceptors 14 |
No. Hydrogen Bond Donors 6 |
Applications
LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (10 ng/mL) using Strata-X and Kinetex C8
19694
Separation Mode: Reversed Phase
Reversed Phase
C8
HPLC
Anti-Arrhythmia Agents
Clinical
Applications
LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (10 ng/mL) using Strata-X and Kinetex C8
Analytes
Details
LC Conditions (App ID: 19694)
Column: | ||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||
Part No: | ||||||||||||||||||||
Phase Name: C8 | ||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||
Mobile Phase: A: 10mM Ammonium acetate B: 10mM Ammonium acetate in methanol | ||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||
Flow Rate: 400 µL/min | ||||||||||||||||||||
Temperature: 0°C | ||||||||||||||||||||
Detection: Mass Spectrometer (MS) (350°C) | ||||||||||||||||||||
Detector Info: SCIEX API 4000™ |
Sample Preparation Details
Description: Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk |
Part Number: |
Condition 1: Methanol |
Condition 2: 10mM Ammonium acetate |
Wash 1: 50:50 10mM ammonium acetate/10mM ammonium acetate |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Condition 10mM Ammonium acetate |
1 Shake Methanol |
1 Wash 50:50 10mM ammonium acetate/10mM ammonium acetate |
Order Items Used in This Application
00B-4497-AN
Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Qty:
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Digoxin
Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
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