| 1Methadone-d9 |
|---|
| 2Methadone |
Compound Name Methadone |
CID 4095 |
Molecular Formula C21H27NO |
Molecular Weight 309 |
No. Chiral Centers: 1 |
No. Hydrogen Bond Acceptors 2 |
Applications
LC/MS/MS Analysis of Methadone from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm
19703
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Forensics/Toxicology
Applications
LC/MS/MS Analysis of Methadone from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm
Analytes
Details
LC Conditions (App ID: 19703)
Column: | ||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||
Part No: | ||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||
Mobile Phase: A: 0.1% Formic acid B: 0.1% Formic acid in acetonitrile | ||||||||||||||||||||
Gradient Profile:
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Flow Rate: 400 µL/min | ||||||||||||||||||||
Temperature: 0°C | ||||||||||||||||||||
Detection: Mass Spectrometer (MS) (22°C) | ||||||||||||||||||||
Detector Info: SCIEX API 3000™
Source: Cur-11, CAD-12, IS-4500, Tem-450, NEB-12
Dwell: 25ms
Polarity: Positive |
Sample Preparation Details
Description: Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk |
Part Number: |
Pre Treatment Note: Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 40% acetonitrile. |
Sample PreTreatment: Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 40% acetonitrile. |
Wash 1: 2 mLs of 100mM sodium acetate buffer (pH 5) |
Wash 2: 2 mLs of Methanol |
Evaporation: Evaporate to dryness |
1 Shake 2mLs of Ethyl acetate:Isopropanol:NH4OH (7:2:1) |
1 Wash 2 mLs of 100mM sodium acetate buffer (pH 5) |
2 Wash 2 mLs of Methanol |
Order Items Used in This Application
00B-4462-AN
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Qty:
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