| 1Testosterone |
|---|
| 2Testosterone-d3 |
| 3Epitestosterone |
Compound Name Testosterone |
CID 6013 |
Molecular Formula C19H28O2 |
Molecular Weight 288 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 1 |
Applications
Separation of Testosterone and Epitestosterone by LC/MS/MS using Strata-X-A and Kinetex 1.7 µm C18
19761
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Androgens
Clinical
Applications
Separation of Testosterone and Epitestosterone by LC/MS/MS using Strata-X-A and Kinetex 1.7 µm C18
Analytes
Details
LC Conditions (App ID: 19761)
Column: | ||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||
Part No: | ||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||
Mobile Phase: A: 0.1% Formic Acid +1 mM Amm Formate in Water B: 0.1% Formic Acid +1 mM Amm Formate in ACN | ||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||
Flow Rate: 0.4 mL/min | ||||||||||||||||||||
Temperature: 0°C | ||||||||||||||||||||
Detection: Mass Spectrometer (MS) (22°C) | ||||||||||||||||||||
Detector Info: SCIEX API 5000™ |
Sample Preparation Details
Description: Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 3 mL, Tubes, 50/Pk |
Part Number: |
Pre Treatment Note: Following evaporation of elution solvent @ 50-55 C under gentle nitrogen stream;
Add 50 uL 25% hydroxylamine solution and heat at 60-65 C for 5-10 min, then add 200 uL 5% aqueous formic acid and vortex the tubes
Transfer the solution to autosampler vials and inject 25 uL on column |
Condition 1: Methanol |
Condition 2: Water |
Sample PreTreatment: Following evaporation of elution solvent @ 50-55 C under gentle nitrogen stream;
Add 50 uL 25% hydroxylamine solution and heat at 60-65 C for 5-10 min, then add 200 uL 5% aqueous formic acid and vortex the tubes
Transfer the solution to autosampler vials and inject 25 uL on column |
Wash 1: 50% Methanol in Water |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Shake Methanol |
1 Condition Methanol |
2 Shake Methanol |
1 Condition Methanol |
Order Items Used in This Application
00A-4475-AN
Kinetex 1.7 µm C18 100 Å, LC Column 30 x 2.1 mm, Ea
Qty:
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