| 1Nornicotine |
|---|
| 23-Hydroxycotinine |
| 3Anabasine |
| 4Cotinine |
| 5Nicotine |
Compound Name Nornicotine |
CID 412 |
Molecular Formula C9H12N2 |
Molecular Weight 148 |
No. Chiral Centers: 1 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 1 |
Applications
Nicotine and metabolites by LCMS using Gemini NX 3u C18 100x2.1mm
20651
Nicotinic Agonists
Clinical
Therapeutic / Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical
Analytes
Details
LC Conditions (App ID: 20651)
Elution Type: Gradient | ||||||||||||||||||||
Mobile Phase: A: 20mM Ammonium Bicarbonate B: Acetonitrile | ||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||
Flow Rate: 0.5 mL/min | ||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) | ||||||||||||||||||||
Detector Info: SCIEX API 4000™ |
Sample Preparation Details
Condition 1: Methanol |
Condition 2: Ammonium acetate buffer |
Wash 1: Ammonium acetate buffer |
Wash 2: 30% Methanol |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Wash 30% Methanol |
1 Shake 1.5% Ammonium hydroxide methanol solution |
2 Wash 30% Methanol |
1 Shake 1.5% Ammonium hydroxide methanol solution |
2 Wash 30% Methanol |
Similar Applications
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Nornicotine
Kinetex 2.6 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, EA
Reversed Phase
Gemini 5 µm C18 110 Å, LC Column 100 x 2 mm, Ea
Reversed Phase
Luna 5 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea
Hydrophilic Interaction
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