| 1Doxepin |
|---|
| 2DM-Doxepin |
| 3Imipramine-d3 |
| 4Imipramine |
| 5Desipramine-d3 |
| 6Desipramine |
| 7Nortriptyline-d3 |
| 8Nortriptyline |
| 9Amitriptyline |
| 10Protriptyline-d3 |
| 11Protriptyline |
| 12Clomipramine-d3 |
| 13Clomipramine |
| 14DM-Clomipramine |
Compound Name Doxepin |
CID 667477 |
Molecular Formula C19H21NO |
Molecular Weight 279 |
No. Hydrogen Bond Acceptors 2 |
Column: | ||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: 0.1% formic acid/water B: 0.1% Formic acid/methanol | ||||||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||||||
Flow Rate: 0.4 mL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Mass Spectrometer (MS) (22°C) | ||||||||||||||||||||||||
Detector Info: SCIEX API 4000™ | ||||||||||||||||||||||||
Sample Note: MS/MS Parameters
Compound Q1 Q3 Dwell time DP EP CE CXP
Protrip 264.2 191.1 35 60 10 35 10
Notrip 264.3 191.2 35 60 10 35 10
DM-Dox 266.2 107.1 25 60 10 35 10
Desip 267.2 208.2 35 60 10 35 10
Protrip-D3 267.2 191.1 35 60 10 35 10
Notrip-D3 267.3 191.2 35 60 10 35 10
Desip-D3 270.2 208.2 35 60 10 35 10
Amitrip 278.2 191.2 35 60 10 35 10
Dox 280.3 107.1 25 60 10 35 10
Imip 281.3 208.2 35 60 10 35 10
Imip-D3 284.2 208.1 35 60 10 35 10
DM-Clomip 301.2 242.2 35 60 10 35 10
Clomip 315.2 242.2 35 60 10 35 10
Clomip-D3 318.2 242.2 35 60 10 35 10
Ionization Parameters
ESI in positive polarity
Gas 1 50
Gas 2 50
CAD 8
Cur 20
IS 5500
Temp 600 |
Description: Strata-X-Drug B 33 µm Polymeric Strong Cation, 30 mg / well, 96-Well Plates, 2/Pk |
Part Number: |
Pre Treatment Note: To do % absolute recovery, the IS was spiked in the initial mobile phase in the reconstitution step |
Sample PreTreatment: To do % absolute recovery, the IS was spiked in the initial mobile phase in the reconstitution step |
Wash 1: 100mM sodium acetate buffer (pH5.0) |
Wash 2: Methanol |
Evaporation: Evaporate to dryness |
1 Shake Methanol/ACN(5/50) + 2% ammonium hydroxide |
1 Wash 100mM sodium acetate buffer (pH5.0) |
2 Wash Methanol |
