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Applications

Human Plasma TMP & Thiamine by Gemini-NX 3u C18 100x3mm with Impact Plate_2.5nmol vs Blank

Analytes

1TMP
2Thiamine

Compound Name Trimethoprim
CID 5578
Molecular Formula C14H18N4O3
Molecular Weight 290
No. Hydrogen Bond Acceptors 7
No. Hydrogen Bond Donors 2

Details

LC Conditions (App ID: 21880)

Column:

Gemini 3 µm NX-C18 110 Å, LC Column 100 x 3 mm, Ea

Brand Name:

Part No:

Phase Name:

NX-C18

Elution Type:

Gradient

Mobile Phase:

A: 25nM Na2HPO4, 10% methanol

B: 25mM Na2HPO4, 70% methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	3	97
2	0.25	25	75
3	0.75	25	75
4	3	35	65
5	4	100	0
6	5	100	0
7	5.1	3	97
8	8	3	97

Flow Rate:

0.75 mL/min

Temperature:

22°C

Detection:

Fluorescence (FLUOR) @ Ex 375 nm (22°C)

Detector Info:

Shimadzu ELSD

Sample Preparation Details

Description: Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part Number: CE0-7565
Evaporation: Evaporate to dryness

Order Items Used in This Application

00D-4453-Y0

Gemini 3 µm NX-C18 110 Å, LC Column 100 x 3 mm, Ea

Qty:

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