For security purposes
FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Applications
FMOC derivatized glyphosate on a Gemini 3 µm NX-C18
22749
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Antifungal Agents

Applications

FMOC derivatized glyphosate on a Gemini 3 µm NX-C18
Analytes
1Glyphosate
22-Aminoethylphosphonic acid (AMPA)
Compound Name
Glyphosate
CID
3496
Molecular Formula
C3H8NO5P
Molecular Weight
169
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4
Details
LC Conditions (App ID: 22749)
Column:
Brand Name:
Part No:
Phase Name:
NX-C18
Elution Type:
Gradient
Mobile Phase:
A: 5 mM Ammonium bicarbonate pH 9
B: ACN/Methanol (50:50)
Gradient Profile:
Step No.Time(min)%A%B
108020
241090
34.18020
478020
Flow Rate:
0.4 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) (22°C)
Detector Info:
API 5000
Sample Note:
Derivatization protocol: In a 2 mL vial, spike 50 µL of 10 µg/mL Glyphosate and AMPA standards into 1 mL of 0.4 M borate buffer. Add 200 µL of 2 mg/mL FMOC in acetone (make fresh daily) and vortex.

Order Items Used in This Application

Similar Applications
search
No Data