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Applications
Steroids mix using Kinetex 5u C18 150x4.6mm
22952
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Inflammatory Agents

Applications

Steroids mix using Kinetex 5u C18 150x4.6mm
Analytes
1Hydrocortisone
2Corticosterone
311-a-Hydroxyprogesterone
4Cortisone acetate
511-Ketoprogesterone
Compound Name
Hydrocortisone
CID
5754
Molecular Formula
C21H30O5
Molecular Weight
362
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 22952)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Isocratic
Mobile Phase:
A: 0.1% H3PO4 in Water
B: Acetontrile
C: Methanol
Gradient Profile:
Step No.Time(min)%A%B%C
10543511
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Diode Array (PDA) @ 254 nm (22°C)

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