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Applications
Cefaclor forced degradation on Kinetex 2.6 µm EVO C18
22988
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Anti-Bacterial Agents
Pharmaceutical/Biopharmaceutical
Pharmaceutical
Therapeutic / Clinical

Applications

Cefaclor forced degradation on Kinetex 2.6 µm EVO C18
Analytes
1Cefaclor
Compound Name
Cefaclor
CID
51039
Molecular Formula
C15H14ClN3O4S
Molecular Weight
367
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 22988)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
EVO C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% H3PO4 in Water
B: 0.1% H3PO4 in ACN
Gradient Profile:
Step No.Time(min)%A%B
10955
266040
Flow Rate:
1.75 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
Forced degradation protocol: Reconstitute 1 mg of cefaclor neat standard with 1.0 mL of 1 M HCl. Heat the solution at 60 ºC for 6 hours then neutralize with 1.0 mL of 1 M NaOH. Refrigerate until ready to inject.

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