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Applications
Green Coffee on Kinetex 2.6u EVO C18 50x2.1mm @ 0.4mL/min
23000
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Food & Beverage
Product / Trade Good
Biochemical Compound / Nutrient

Applications

Green Coffee on Kinetex 2.6u EVO C18 50x2.1mm @ 0.4mL/min
Analytes
1Chlorogenic Acid
Compound Name
Chlorogenic Acid
CID
1794427
Molecular Formula
C16H18O9
Molecular Weight
354
No. Hydrogen Bond Acceptors
9
No. Hydrogen Bond Donors
6
Details
LC Conditions (App ID: 23000)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
EVO C18
Elution Type:
Gradient
Mobile Phase:
A: 20mM Potassium Phosphate pH 2.5
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
253070
363070
46.5955
510955
Flow Rate:
0.4 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)

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