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Applications
Vitamin A and E from infant formula (Gerber) on Luna NH2 by UV
23376
Separation Mode: Normal Phase
Normal Phase
NH2
HPLC
Adjuvants, Immunologic

Applications

Vitamin A and E from infant formula (Gerber) on Luna NH2 by UV
Analytes
1Vitamin A acetate (isomer)
2Vitamin A acetate
Compound Name
Retinol Acetate
CID
638034
Molecular Formula
C22H32O2
Molecular Weight
328
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 23376)
Column:
Brand Name:
Part No:
Phase Name:
NH2
Elution Type:
Gradient
Mobile Phase:
A: Hexane
B: 50 % MTBE in Hexane
Gradient Profile:
Step No.Time(min)%A%B
101000
231000
380100
4120100
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 325 nm (22°C)
Sample Note:
Sample prepared following AOAC official method 2012.10 using "Gerber good start" infant formula powder

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