| 1Triphenylene (I.S.) |
|---|
| 2Vitamin D3 |
Compound Name Triphenylene |
CID 9170 |
Molecular Formula C18H12 |
Molecular Weight 228 |
Applications
Vitamin D tablet assay (Naturemade 2000 IU + 100 µg std add) on a Kinetex 2.6 µm Biphenyl, 150 x 4.6
23497
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Vitamins
Applications
Vitamin D tablet assay (Naturemade 2000 IU + 100 µg std add) on a Kinetex 2.6 µm Biphenyl, 150 x 4.6
Analytes
Details
LC Conditions (App ID: 23497)
Column: | ||||||||
Brand Name: Kinetex | ||||||||
Part No: | ||||||||
Phase Name: Biphenyl | ||||||||
Elution Type: Isocratic | ||||||||
Mobile Phase: A: 0.1% TFA B: Acetonitrile | ||||||||
Gradient Profile:
| ||||||||
Flow Rate: 1.75 mL/min | ||||||||
Temperature: 40°C | ||||||||
Detection: UV-Vis Abs.-Diode Array (PDA) @ 280 nm (22°C) | ||||||||
Sample Note: Sample preparation: small tablets (<400 mg)
1. Grind tablet into powder and transfer to a 2 mL dSPE tube (KS0-8916)
2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable)
3. Add 800 µL of water
4. Sonicate until dissolved (approx. 5 min)
5. Add 800 µL of acetonitrile
6. Shake for 10 min using mechanical shaker
7. Centrifuge at 15000 rpm for 3 min
8. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis
Sample preparation: large tablets (>400 mg)
1. Grind tablet into powder and transfer to a 5 mL centrifuge tube
2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable)
3. Add 2 mL of water
4. Sonicate until dissolved (approx. 5 min)
5. Add 400 mg of Quechers salt (AH0-9044)
6. Add 2 mL of acetonitrile
7. Shake for 10 min using mechanical shaker
8. Centrifuge at 6000 rpm for 3 min
9. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis |
Order Items Used in This Application
00F-4622-E0
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 150 x 4.6 mm, Ea
Qty:
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Triphenylene (I.S.)
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