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Applications
EtG / EtS from Urine on Luna Omega 5u Polar C18, 50x4.6mm
23842
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Forensics/Toxicology
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

EtG / EtS from Urine on Luna Omega 5u Polar C18, 50x4.6mm
Analytes
1Interference
2Ethyl Sulfate (EtS)
3Ethyl Glucuronide (EtG)

Analyte not found

Details
LC Conditions (App ID: 23842)
Column:
Brand Name:
Part No:
Phase Name:
Polar C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid in H2O
B: 0.1% Formic Acid in MeOH
Gradient Profile:
Step No.Time(min)%A%B
10982
224060
32.011090
431090
53.01982
64982
Flow Rate:
1 mL/min
Temperature:
25°C
Detection:
Tandem Mass Spec (MS-MS) (550°C)
Detector Info:
SCIEX 4000 QTRAP®
Sample Note:
MMA (117.0-->72.9; 117.0-->54.9) MMA-D3 (120.1-->75.9)
Sample Preparation Details
Description:
Strata-X-AW 33 µm Polymeric Weak Anion, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:
Condition 1:
Methanol
Condition 2:
0.1% Acetic Acid in Water
Wash 1:
50:50 Methanol:Water
Evaporation:
Evaporate to dryness
1
Condition
Methanol
2
Shake
2% Ammonium Hydroxide in Methanol
1
Condition
Methanol
2
Shake
2% Ammonium Hydroxide in Methanol
1
Condition
Methanol

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