| 1Diazepam |
|---|
| 2D5-Nordiazepam |
| 3Nordiazepam |
| 4D5-Oxazepam |
| 5Oxazepam |
| 6D5-Temazepam |
| 7Temazepam |
| 8Clonazepam |
| 9Lorazepam |
| 10Alprazopam |
| 11D5-alpha-Hydroxyalprazolam |
| 12Alpha-Hydroxyalprazolam |
Compound Name Diazepam |
CID 3016 |
Molecular Formula C16H13ClN2O |
Molecular Weight 284 |
No. Hydrogen Bond Acceptors 2 |
Injection: Split 20:1 @ 260°C, 1.0 μL |
Oven Program: 200 to 340 over 25C/min hold for 1 minute |
Carrier Gas: Constant Flow Helium, 17.2 mL/min |
Detection: Mass Spectrometer (MS) (320°C) |
Description: Strata Screen-C (55 µm, 70 Å), 150 mg / 3 mL, Tubes, 50/Pk |
Part Number: |
Pre Treatment Note: Reconstitute in 100uL Ethyl Acetate and 50uL MTBSTFA; Cap and incubate @ 60C for 20minutes then transfer to amber vial with insert. Inject 1uL. |
Condition 1: Methanol |
Condition 2: 100mM Potassium Phosphate pH 6.0 |
Sample PreTreatment: Reconstitute in 100uL Ethyl Acetate and 50uL MTBSTFA; Cap and incubate @ 60C for 20minutes then transfer to amber vial with insert. Inject 1uL. |
Wash 1: 100mM Potassium Phosphate pH 6.0 |
Wash 2: 20:80 (100mM Potassium Phosphate pH 6.0 : Methanol) |
Wash 3: Hexane |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Wash 20:80 (100mM Potassium Phosphate pH 6.0 : Methanol) |
1 Shake Ethyl Acetate:Isopropanol:Ammonium Hydroxide (70:20:10) |
1 Wash 100mM Potassium Phosphate pH 6.0 |
2 Condition 100mM Potassium Phosphate pH 6.0 |
3 Wash Hexane |
