| 1C16:0 |
|---|
| 2C18:1 cis 9 |
| 3C18:2 cis 9,12 |
| 4C18:3 cis 9,12,15 |
Compound Name Palmitic Acid |
CID 985 |
Molecular Formula C16H32O2 |
Molecular Weight 256 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 1 |
Applications
FAMEs In Canola Oil by GC/MS on Zebron ZB-FAME
24074
Separation Mode: Application Specific
Application Specific
ZB-FAME
GC
Enzyme Inhibitors
Food & Beverage
Analytes
Details
GC Conditions (App ID: 24074)
Column: |
Part No: |
Phase Name: ZB-FAME |
Injection: Split 50:1 @ 240°C, 1.0 μL |
Oven Program: 100 °C for 2 min to 214 °C @ 3 °C/min |
Carrier Gas: Constant Flow Helium, 1.0 mL/min |
Detection: Mass Spectrometer (MS) (250°C) |
Sample Notes: Recommended Accessories:
Recommended Liner: Zebron PLUS Single Taper Z-Liner, 4 mm ID
Liner Part No.: AG2-0A13-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)
Sample Preparation:
1. Weigh out 25 mg of oil into a 5 mL screw cap glass vial
2. Add 2 mL of BF3-methanol to sample vial
3. Heat vial at 60 °C for 15 minutes with periodic vortexing
4. Remove from heat and allow to attain room temperature
5. Add 1 mL water followed by 1 mL hexane
6. Vortex for 1 minute
7. Remove upper organic layer
8. Dry hexane layer over anhydrous sodium sulfate
9. Transfer to autosampler vial and inject on GC |
Order Items Used in This Application
7HG-G033-10
Zebron ZB-FAME, GC Cap. Column 30m x 0.25mm x 0.2 µm, Ea
Qty:
Similar Applications
search
C16:0
No Data
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
CONNECT WITH US
