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Applications
Water-Soluble Vitamins
2582
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Vitamin B Complex
Pharmaceutical
Therapeutic / Clinical

Applications

Water-Soluble Vitamins
Analytes
1Vitamin B12
2Folic acid
3Biotin

Analyte not found

Details
LC Conditions (App ID: 2582)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: 90% 0.05 M Hexane sulfonic acid, 0.1% phosphoric acid in methanol
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210-280 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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