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Applications
Lidocaine HCl - Alternative Method
3300
Separation Mode: Reversed Phase
Reversed Phase
USP L07
C8(2)
HPLC
Preservatives, Pharmaceutical
Pharmaceutical
Therapeutic / Clinical

Applications

Lidocaine HCl - Alternative Method
Analytes
1Lidocaine
2Methyl paraben
Compound Name
Lidocaine
CID
3676
Molecular Formula
C14H22N2O
Molecular Weight
234
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
Details
Official Method: USP L07
LC Conditions (App ID: 3300)
Column:
Brand Name:
Part No:
Phase Name:
C8(2)
Elution Type:
Isocratic
Mobile Phase:
A: 20mM Ammonium acetate with 30mM TFA
B: Acetonitrile
C: MeOH
Gradient Profile:
Step No.Time(min)%A%B%C
10701020
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282

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