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Applications
Water-soluble vitamins
3305
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Vitamin B Complex
Pharmaceutical
Therapeutic / Clinical

Applications

Water-soluble vitamins
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Analytes
1Thiamine
2Cyanocobalamine (Vitamin B12)
3Ascorbic acid
4Pantothenic acid
5Niacinamide
6paraaminobenzoic acid
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1
Details
LC Conditions (App ID: 3305)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: 20mM Potassium phosphate
B: ACN
Gradient Profile:
Step No.Time(min)%A%B
10955
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)

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