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Applications
Water-Soluble Vitamins
5292
Vitamin B Complex
Pharmaceutical
Therapeutic / Clinical

Applications

Water-Soluble Vitamins
Analytes
1Ascorbic acid
2Nicotinic acid
3Nicotinamide
4Calcium pantothenate
5Pyridoxal hydrochloride
6Pyridoxine hydrochloride
7Pyridoxamine dihydrochloride
8Thiamine hydrochloride
9Folic acid
10Biotin
11Riboflavin
Compound Name
Ascorbic Acid
CID
5785
Molecular Formula
C6H8O6
Molecular Weight
176
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4
Details
LC Conditions (App ID: 5292)
Elution Type:
Isocratic
Mobile Phase:
A: Acetonitrile
B: 5mM 1-Hexanesulfonic acid sodium salt + 20mM phosphoric acid (pH 2.3)
Gradient Profile:
Step No.Time(min)%A%B
10892
Flow Rate:
1 mL/min
Temperature:
40°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282
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