Applications

Proteins II

Analytes

1Alkaline phosphatase
2Cyanocobalamin
3RNAse
4Insulin
5Transferrin
6Trypsin inhibitor

Analyte not found

Details

LC Conditions (App ID: 5370)

Column:

Jupiter 5 µm C4 300 Å, LC Column 50 x 4.6 mm, Ea

Brand Name:

Jupiter

Part No:

00B-4167-E0

Phase Name:

Elution Type:

Gradient

Mobile Phase:

A: 0.1% TFA in water

B: 0.1% TFA in acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	100	0
2	1	80	20
3	2.5	65	35
4	4	54	46
5	6	54	46

Flow Rate:

1 mL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 220 nm (22°C)

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C4 4 x 3.0mm, 10/Pk Part No.: AJ0-4330 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00B-4167-E0

Jupiter 5 µm C4 300 Å, LC Column 50 x 4.6 mm, Ea

Qty:

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