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Applications
Dabsyl Derivated Phospho Amino Acids
5642
Separation Mode: Reversed Phase
Reversed Phase
ODS (30)
HPLC
Glycine Agents
Clinical Research
Therapeutic / Clinical

Applications

Dabsyl Derivated Phospho Amino Acids
Analytes
1Phosphothreonine
2Phosphoserine
3Phosphotyrosine
4Aspartic acid
5Glutamic acid
6Carboxymethylcysteine
7Serine
8Threonine
9Glycine
10Alanine
11Proline
12Valine
13Methionine
14Isoleucine
15Leucine
16Arginine
17Phenylalanine
18Cysteine
19Ammonia
20Lysine
21Histidine
22Dityrosine
23Tyrosine
Compound Name
Phosphothreonine
CID
1016
Molecular Formula
C4H10NO6P
Molecular Weight
199
No. Chiral Centers:
2
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
4
Details
LC Conditions (App ID: 5642)
Column:
Brand Name:
Part No:
Phase Name:
ODS (30)
Elution Type:
Gradient
Mobile Phase:
A: 20 mM Sodium bicarbonate pH 8.1 with 4% dimethylformamide
B: (90:10) Acetonitrile/2-Propanol
Gradient Profile:
Step No.Time(min)%A%B
108218
267525
3266040
4413070
5433070
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 460 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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