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Our reversed phase HPLC Column Match web tool allows you to develop reversed methods without having to go on the lab. Quickly and easily reversed HPLC column develop methods based on compound type, USP methods, application, or desired column phase. So take off you lab coat, relax and let our web tool do the work.
Being the most common principle HPLC/UHPLC separation mode, reversed phase chromatography offers dynamic retention of compounds with hydrophobic and organic functionality. Retention of these compounds by reversed phase involves a combination of hydrophobic and van der Waals type interactions between each target compound and both the stationary phase and mobile phase.
Stationary phases used in reversed phase chromatography typically consist of varying lengths of hydrocarbons such as C18, C8, and C4 or strongly hydrophobic polymers such as styrene divinylbenzene.
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Normal phase chromatography (NPC) is used to separate hydrophobic compounds and matrices that are retained too strongly by reversed phase and have minimal solubility in aqueous mobile phases.
Normal phase stationary phases typically include polar functional groups (silica, amino, and cyano) and interact with analytes primarily via dipole-dipole and hydrogen bonding interactions.
More information on the entire Luna HPLC column line
HILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest.
HILIC HPLC/UHPLC columns draw and retain a water-enriched layer onto the surface of the silica which facilitates the interaction of polar compounds with the stationary phase for increased retention.
Tips and techniques for optimal HILIC method construction
Using Strata® Melamine SPE and Ultra-fast LC/MS/MS Analysis Using Kinetex™ HILIC
Using Strata-X-CW and LC/MS/MS on Luna HILIC
Create more favorable conditions for polar compound retention and ionization
Ion-exchange (IEX) chromatography involves interactions between a charged stationary phase and the oppositely charged mobile analytes. In cation-exchange chromatography positively charged molecules are attracted to a negatively charged stationary phase. Likewise, in anion-exchange chromatography negatively charged molecules are attracted to a positively charged stationary phase.
Ion-exchange (IEX) chromatography is useful for a wide variety of compounds, such as acidic (anionic) and basic (cationic) small molecules up to peptides and proteins.
Reversed Phase & Ion-Exchange Columns for high purity synthetic oligonucleotide purification
Optimizing Sensitivity and Selectivity for the Analysis and Characterization of Synthetic Oligonucleotides via LC/MS
Ion exclusion (IEC) chromatography is a process of separating components in a mixture by means of an ion-exchange resin that excludes highly ionized particles and retains slightly ionized or non-ionized particles. Ion exclusion (IEC) chromatography applies to the retention of organic acids, carbohydrates, sugars, starches, and oligosaccharides, using a sulfonated stationary phase.
From drug formulation and excipient analysis to quality control testing of finished food products to fermentation monitoring of bioethanol production, ion exclusion provides the necessary accurate and reproducible analytical results.
Reproducible Separation of Carbohydrate, Oligosaccharide, and Organic Acid Analysis
Quickly monitor bioethanol fermentation broths and microbial growth with Rezex ion exclusion HPLC columns
Faster Real-time Response to Bacterial Infection of Bioethanol Fermentation using a Short Rezex ROA Column
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