HPLC


Ultra-High Performance on ANY LC System

Kinetex ® core-shell 2.6 µm and 1.7 µm particles were engineered to provide increased efficiencies and improved performance compared to traditional fully porous particles.

  • Increase resolution, throughput, and sensitivity
  • Decrease solvent consumption
  • Save time and money
  • 1.7 µm and 2.6 µm particles are directly scalable
  • Available in C18, XB-C18, C8, PFP, and HILIC phases

2.6 µm Kinetex columns on your HPLC or UHPLC
systems provide sub-2 µm efficiencies with significantly lower back pressure.


1.7 µm Kinetex columns »on your UHPLC system provide the highest efficiencies of any sub-2 µm column for breakthrough performance.

See Kinetex in Action on your System


select a phase:

HILIC phase information

HILIC phase detail

This unbonded silica phase is used under hydrophilic interaction chromatography conditions to provide the selectivity needed for the retention and separation of polar compounds.




Internal Diameter: Length:

Technical Specifications:

Phase Particle Sizes (µm)Pore Size (Å)Surface Area (sq. m/g)Carbon Load (%) pH Range Recommended Use

Technical Documents:

Document Type:
document type title
Brochures Kinetex 2.6 µm and 1.7 µm brochure

Summary

Acheive High performance anywhere with Kinetex 2.6 µm and 1.7 µm particles

Brochures Ultra-High Performance on Any LC System

Summary

Make any HPLC system perform like a UHPLC system with Kinetex™ core-shell technology columns

Journals Performance of columns packed with the new shell Kinetex-C18 particles in gradient elution chromatography

Summary

The performance of columns packed with the new 2.6 m Kinetex-C18 shell particles was investigated in gradient elution chromatography and compared with those of the 2.7 µm Halo-C18 shell particles and the 1.7 m BEH-C18 totally porous particles.

Journals Performance of columns packed with the new shell particles, Kinetex-C18

Summary

The performance of the new Kinetex-C18 column was investigated. Packed with a new brand of porous shell particles, this column has an outstanding efficiency. Once corrected for the contribution of the instrument extra column volume, the minimum values of the reduced plate heights...

Supporting Docs Agilent 1100 Optimization Video

Summary

Demonstration of how Kinetex core-shell technology HPLC columns can be used on an Agilent 100 system to achieve maximum performance and optimal results

Supporting Docs Kinetex Scaling Guide

Summary

The following guide outlines steps to achieving Ultra-High Performance using Kinetex core-shell technology columns

Supporting Docs Kinetex Selectivity Poster

Summary

Outlines guidelines for selecting the right Kinetex column for your application

Technical Notes Increased Efficiency and Resolution with Kinetex Core-Shell Technology

Summary

The combination of the small particle size and narrow particle size distribution coupled with the significantly shorter diffusion path provided by the Kinetex™ core-shell particle results in a material that yields significantly increased column efficiencies and chromatographic resolution. This is a significant benefit for separation scientists looking to increase efficiency and chromatographic resolution, especially for complex separations containing many compounds and very closely eluting compounds so that accurate identification and quantitation can be achieved.

Technical Notes Rapid LC/MS/MS Analysis of Antibiotics in Meat

Summary

The method described here is for screening of seven classes of antibiotics: beta-lactam, tetracycline, sulfonamide, macrolide, amphenicol, fluoroquinolone, and flunixin. HPLC method development issues are explored and a newly introduced high efficiency core-shell particle is used as HPLC media to optimize retention and provide higher sensitivity of analysis

Brochures Kinetex 2.6 µm and 1.7 µm brochure

Summary

Acheive high performance anywhere with Kinetex 2.6 µm and 1.7 µm particles

Supporting Docs HILIC Method Development Guidelines

Summary

Outline basic guidelines and considerations for HILIC method development

Supporting Docs Installing Kinetex Columns on an Acquity UHPLC System

Summary

Installing Kinetex Columns on an Acquity UHPLC System

Supporting Docs Significant Costs Savings Achieved Through the Development of a New Analytical Method for Residual API Analysis, with a Kinetex 1.7 μm Core-Shell UHPLC Column

Summary

Significant Costs Savings Achieved Through the Development of a New Analytical Method for Residual API Analysis, with a Kinetex 1.7 μm Core-Shell UHPLC Column

Technical Notes An Improved HPLC Method for the Determination of Ranitidine Suitable for All Dosage Forms using Kinetex® Core-Shell Technology Columns

Summary

An Improved HPLC Method for the Determination of Ranitidine Suitable for All Dosage Forms using Kinetex® Core-Shell Technology Columns

Technical Notes Determination of Impurities and Related Substances for Metoprolol Tartrate (Ph. Eur. Monograph 1028): Increased Sensitivity, Improved Resolution and Faster Analysis Using Kinetex™ 2.6 μm Core-Shell LC Columns

Summary

Determination of Impurities and Related Substances for Metoprolol Tartrate (Ph. Eur. Monograph 1028): Increased Sensitivity, Improved Resolution and Faster Analysis Using Kinetex™ 2.6 μm Core-Shell LC Columns

Technical Notes Enhancing Sensitivities and Peak Capacities for UHPLC-MS Fast Gradient Analyses

Summary

Enhancing Sensitivities and Peak Capacities for UHPLC-MS Fast Gradient Analyses When Exploiting the Properties of 2.6 μm Kinetex® Core-Shell Columns

Technical Notes Evaluation of Newer Technologies for the LC/MS/MS Analysis of Amphetamines using Strata-X-Drug B Solid Phase Extraction (SPE) and Kinetex Core-Shell HPLC/UHPLC Columns

Summary

Evaluation of Newer Technologies for the LC/MS/MS Analysis of Amphetamines using Strata™-X-Drug B Solid Phase Extraction (SPE) and Kinetex® Core-Shell HPLC/UHPLC Columns

Technical Notes Improved Analytical Method for the Analysis of Chloramphenicol in Shrimp and Other Marine Food Products

Summary

Improved Analytical Method for the Analysis of Chloramphenicol in Shrimp and Other Marine Food Products

Technical Notes Improved LC/MS/MS Analysis of Gabapentin in Plasma using a Kinetex

Summary

Improved LC/MS/MS Analysis of Gabapentin in Plasma using a Kinetex® 2.6 μm Core-Shell C18 Column following Protein Precipitation

Technical Notes Increased Sensitivity, Improved Resolution and Faster Analysis Times For Compendial Methods

Summary

Recently, a newly developed Kinetex™ 2.6 μm core-shell chromatographic particle has been commercialized that offers the performance benefits of sub-2 μm fully-porous particles but at substantially lower operating pressures. To demonstrate the performance benefits of this new core-shell technology, a Kinetex™ 2.6 μm core-shell C18 column was compared with a fully porous 5 μm C18 column referenced in EP ([Ph. Eur.] European Pharmacopoeia) Monograph 0703 for Atenolol and related substances on a conventional HPLC instrument with an upper pressure limit of 400 bar

Technical Notes Increased Speed of Analysis and Sample Throughput with Kinetex Core-Shell Technology

Summary

Increased Speed of Analysis and Sample Throughput with Kinetex Core-Shell Technology

Technical Notes Rapid Analysis of Hop Acids in Beer

Summary

Rapid Analysis of Hop Acids in Beer using Strata™-X Solid Phase Extraction and Kinetex® 2.6 μm Core-Shell Technology Column

Technical Notes Rapid, High Resolution Analysis of Aflatoxin Extracts from Peanut Butter Using Kinetex™ Core-Shell Technology and Strata® SPE

Summary

A two-stage Solid Phase Extraction (SPE) procedure using Strata® Florisil® and Strata® Silica is effective in removing detector interfering contaminants from the difficult sample matrix, peanut butter, while maintaining absolute recoveries above 80 %. Improved separation of aflatoxins is achieved with Kinetex™ 2.6 μm PFP core-shell columns, compared to traditional C18 columns, allowing for rapid run times under 2 minutes with great precision, and accuracy.

Brochures Better UHPLC Performance Starts NOW with Kinetex 1.7 µm Core-Shell Columns

Summary

Better UHPLC Performance Starts NOW with Kinetex 1.7 µm Core-Shell Columns

Journals Comparative study of new shell-type, sub 2 µm fully porous and monolith stationary phases, focusing on mass-transfer resistance

Summary

Today sub-2 µm packed columns are very popular to conduct fast chromatographic separations. The mass transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass transfer resistance...

Technical Notes Complementary Selectivities of Kinetex Core-Shell Phases

Summary

Further research and product development of Kinetex core-shell media has culminated with the introduction of two additional bonded phases, Kinetex XB-C18 and Kinetex C8. These new phases offer complementary selectivities to the existing Kinetex phases (C18, PFP and HILIC) giving chromatographers additional solutions for their ultra-high performance separations. This technical note will cover some general observations regarding the selectivity of the new Kinetex

Technical Notes Determination of Impurities and Related Substances for Acetylsalicylic Acid

Summary

Recently, a newly developed Kinetex 2.6 μm core-shell chromatographic particle has been commercialized that offers the performance benefits of fully porous sub-2 μm particles but at substantially lower operating pressures. To demonstrate the performance benefits of this new core-shell technology, a Kinetex 2.6 μm core-shell C18 column was compared with a fully-porous 5 μm C18 column referenced in European Pharmacopoeia [Ph. Eur.] Monograph 0309 for Acetylsalicylic acid and related substances on a conventional HPLC instrument with an upper pressure limit of 400 bar

Technical Notes Determination of Impurities and Related Substances for Glibenclamide (EP Monograph 0718)

Summary

Determination of Impurities and Related Substances for Glibenclamide (EP Monograph 0718). Increased Sensitivity, Improved Resolution and Faster Analysis Using Kinetex™ 2.6 μm Core-Shell LC Columns

Technical Notes Development of a High-Throughput LC MS MS Assay for 13 Commonly Prescribed Pain Management Drugs from Urine with Cleanup

Summary

Development of a High-Throughput LC MS MS Assay for 13 Commonly Prescribed Pain Management Drugs from Urine with Cleanup

Technical Notes Evaluation of New HPLC Technologies for the Separation of Preservatives

Summary

Evaluation of New HPLC Technologies for the Separation of Preservatives

Technical Notes Evaluation of Newer HPLC and UHPLC Column Technologies for the Analysis of Steriods

Summary

Evaluation of Newer HPLC and UHPLC Column Technologies for the Analysis of Steriods

Technical Notes Extend Kinetex™ Core-Shell Column Lifetime with KrudKatcher™ Ultra In-Line Filters

Summary

Extend Kinetex™ Core-Shell Column Lifetime with KrudKatcher™ Ultra In-Line Filters

Technical Notes Food Safety Analysis: LC MS MS Applications Using New Kinetex Core-Shell Technology HPLC Columns

Summary

Food Safety Analysis: LC MS MS Applications Using New Kinetex Core-Shell Technology HPLC Columns

Technical Notes High Efficiency Analysis of Testosterone

Summary

High Efficiency Analysis of Testosterone using Kinetex Core-Shell Columns

Technical Notes Improved Throughput and Performance for the USP Propranolol Hydrochloride Assay using a Kinetex® 2.6 μm C8 Core-Shell Column

Summary

The introduction of a new C8 phase to the Kinetex core-shell technology family allows for the ultra-high efficiency, speed and throughput benefits to be applied to methods requiring the use of a C8 (USP L7) column. The application of this technology to the USP assay for propranolol hydrochloride is shown to reduce analysis time while meeting the system suitability requirements for this USP monograph.

Technical Notes Improved Throughput, Productivity, and Performance for the USP Assay for Norethindrone and Mestranol using Kinetex 2.6 µm C8 Core-Shell Columns

Summary

The ultra-high efficiency provided by Kinetex 2.6 μm C8 core-shell columns is utilized to reduce analysis times and increase productivity for the USP assay for norethindrone and mestranol. Several alternatives to shorter column lengths and adjustments to flow rate were used to illustrate how the dramatic increase in efficiency can be leveraged to provide a balance between increasing productivity and reducing solvent usage.

Technical Notes Kinetex 2.6 μm XB-C18 Core-Shell Column for the Rapid, Ultra-High Efficiency USP Assay for Ibuprofen

Summary

Kinetex 2.6 μm XB-C18 Core-Shell Column for the Rapid, Ultra-High Efficiency USP Assay for Ibuprofen

Technical Notes Ultra-fast LC/MS/MS Analysis of 25-OH Vitamin D2 and D3 from Serum

Summary

This technical note describes a sensitive, rugged and robust, highthroughput method for the analysis of 25-OH vitamin D2 and 25-OH vitamin D3 in serum. Following protein precipitation to eliminate proteins and disrupt serum protein binding of 25-OH vitamin D2 and 25-OH vitamin D3, the chromatographic separation of 25-OH vitamin D2 and 25-OH vitamin D3 is carried out in less than 5 minutes using a Kinetex® 2.6 μm C18 core-shell column

Technical Notes Using Core-Shell Kinetex® XB-C18 HPLC Columns as a Solution for Analyzing Fluoroquinolone Antibiotics by LC/MS

Summary

Kinetex XB-C18 is used for developing a rapid LC/MS method for separating fluoroquinolones; when compared to other competitive phases, the Kinetex XB-C18 demonstrates good peak shape and separation of closely related compounds.

Journals Achieving the full performance of highly efficient columns by optimizing conventional benchmark high-performance liquid chromatography instruments

Summary

A series of experiments and measurements demonstrate the importance of minimizing the extra-column band broadening contribution of the instrument used. The combination of several measures allowed the achievement of the full potential efficiency of three Kinetex-C18 columns...

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