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Size Exclusion Columns

Featured SEC HPLC/UHPLC Columns

Yarra Columns

Yarra Aqueous SEC GFC Columns

  • Provides Ultra-high resolution for biomolecules
  • Inert 1.8 µm particle for aggregates, ADCs, mAbs, and biosimilars
  • Economical alternative to BEH SEC columns for HPLC/UHPLC
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Phenogel GPC Columns

Phenogel Non-Aqueous GPC Columns

  • Achieve high resolution and tight linear calibration curves
  • Available in 7 pore sizes from 50 Å to 10E6 Å and a linear mixed bed configuration
  • Temperature stable up to to 140° C
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Separate Biomolecules and Polymers

A Quick Look at (SEC)

Size Exclusion Chromatography (SEC) is a chromatographic method which separates analytes solely based on their size, where molecules are separated on the basis of their exclusion from pores in the column packing material. Larger analytes will elute first, while the smaller molecules interact more with the stationary phase and will elute later.

 

Based on the analytes and the mobile phases used, size exclusion chromatography can be broken into two main categories, aqueous size exclusion/gel filtration chromatography (GFC) and non-aqueous size exclusion/gel permeation chromatography (GPC).

Aqueous Size Exclusion/ Gel Filtration Chromatography (GFC) Analyze and/or characterize proteins, peptides, and other biomolecules such as antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting.

Non-Aqueous Size Exclusion/ Gel Permeation Chromatography (GPC)
Analyze polymers such as adhesives, oils, plasticizers, plastics, resins, and rubbers/elastomers.

Featured Gel Filtration Columns

Yarra Columns

Yarra Aqueous SEC GFC Columns

  • Provides Ultra-high resolution for biomolecules
  • Available in 1.3 µm, 3 µm, 5 µm
  • Ideal for purification, desalting, and characterization
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Separate Biomolecules

Gel Filtration Chromatography (GFC)

Gel filtration chromatography (GFC) is used to separate large macromolecules such as antibodies, immunoglobulins, protein complexes, protein aggregates, peptides, and other biomolecules. In gel filtration chromatography, the compounds of interest “filter” through the stationary phase based on their size.

 

Typically aqueous solvents are used in the mobile phase to ensure that the compound of interest maintains biological activity. Gel filtration columns can separate biomolecules that range from 200 to 1,500,000 Daltons in size. Gel filtration chromatography columns require a GFC column that has low surface activity, high efficiency, and consistent uniform pore size.

Featured Gel Permeation Columns

Phenogel GPC Columns

Phenogel Non-Aqueous GPC Columns

  • Achieve high resolution and tight linear calibration curves
  • Temperature stable up to to 140° C
  • Available in 5 µm and 10 µ particle sizes
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Reduce Solvent for GPC Analysis

The GPC Solvent Calculator

Separate Polymers

A Quick Look at GPC

Gel Permeation Chromatography (GPC) is an effective means of separating and analyzing polymers, resins, oils, plastics, plasticizers, and small organics whose molecular weight can vary from 100-10,000,000 Daltons. HPLC columns used for GPC are packed with polymeric media that can range in pore size from 50 Å to 10E6 Å and can be used with organic solvents such as toluene, THF, methanol, DMF, and methylene chloride.

 

Ideal gel permeation chromatography requires a GPC column that has superior mechanical strength and extreme solvent and temperature compatibility.