Applications

Ion-Pairing pH 8.3 LC/MS Oligonucleotides

Analytes

1poly dT (12-18)

Compound Name Thymidylic Acid
CID 9700
Molecular Formula C10H15N2O8P
Molecular Weight 322
No. Hydrogen Bond Acceptors 8
No. Hydrogen Bond Donors 4

Details

LC Conditions (App ID: 17967)

Column:

Clarity 3 µm Oligo-RP, LC Column 100 x 2 mm, Ea

Brand Name:

Clarity

Part No:

00D-4441-B0

Phase Name:

Oligo-RP

Elution Type:

Gradient

Mobile Phase:

A: 200mM HFIP/14mM TEA 2% MeOH

B: 200mM HFIP/14mM TEA 98% MeOH

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	95	5
2	5	90	10
3	15	85	15
4	60	70	30

Flow Rate:

0.25 mL/min

Temperature:

50°C

Detection:

Mass Spectrometer (MS) (365°C)

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-8134 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00D-4441-B0

Clarity 3 µm Oligo-RP, LC Column 100 x 2 mm, Ea

Qty:

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