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IntantPC Labeled N-Linked Glycans on Trastuzumab, Innovator on bioZen Glycan

Application Detail (App ID: 25039)

Part No:00F-4773-AN
Elution Type:Gradient
Elution A:100 mM Ammonium Formate, pH 4.5
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 22 78
2 10 25.5 74.5
3 24 28 72
4 38.5 44.1 55.9
5 38.6 60 40
6 40.6 60 40
7 40.7 22 78
8 48 22 78

Flow Rate:0.5 mL/min
Col. Temp: 50 °C
Detection: LC/FLD (Fluorescence Detector) @ 285.0000000000 nm (nanometers) (0°C)
Note:Utilize bioZen N-Glycan Clean-Up microelution SPE 96well plate for sample preparation (Part No.: 8M-S009-NGA).

Analyte Details

Analyte Detail not available

1
G0-GlcNAc
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2
G0F-GlcNAc
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3
G0
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4
Man5
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5
G0F
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6
G1F-GlcNAc
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7
G1F
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8
G1F'
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9
G2F
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10
Sialylated Glycan
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11
Sialylated Glycan
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12
Sialylated Glycan
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13
Sialylated Glycan
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