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Applications
Diclofenac Sodium Ph.Eur. Reference Solution B System Suitability on Luna 5 µm C18(2) 250x4.6mm LC/UV
26249
Separation Mode: Reversed Phase
Reversed Phase
Ph.Eur. M.1002
C18(2)
HPLC
Pharmaceutical/Biopharmaceutical
Pharmaceutical
Therapeutic / Clinical

Applications

Diclofenac Sodium Ph.Eur. Reference Solution B System Suitability on Luna 5 µm C18(2) 250x4.6mm LC/UV
Analytes
1Diclofenac Impurity A
2Diclofenac Impurity F
3Diclofenac
Compound Name
Chemdiv3_002423
CID
27211
Molecular Formula
C14H9Cl2NO
Molecular Weight
278
No. Hydrogen Bond Acceptors
1
Details
Official Method: Ph.Eur. M.1002
LC Conditions (App ID: 26249)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: Mix 34 volumes of a solution containing 0.5 g/L of phosphoric acid R and 0.8 g/L of sodium dihydrogen phosphate R, previously adjusted to pH 2.5 with phosphoric acid R, and 66 volumes of methanol R.
Gradient Profile:
Step No.Time(min)%A
140100
Flow Rate:
1 mL/min
Temperature:
25°C
System:
Agilent Technologies Agilent Technologies
Detection:
LC/UV (DAD, PDA) @ 254.0000000000 nm (nanometers)

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