Applications

Lisinopril Ph. Eur. System Suitability Reference Solution A on Kinetex 5 µm C18 250 x 4.6 mm on Agilent 1100 LC/UV

Analytes

1Impurity A
2Lisinopril
3Impurity E

Compound Name Benzylalanine
CID 102530
Molecular Formula C10H13NO2
Molecular Weight 179
No. Chiral Centers: 1
No. Hydrogen Bond Acceptors 3
No. Hydrogen Bond Donors 2

Details

Official Method: Ph.Eur. M.1120

LC Conditions (App ID: 26295)

Column:

Kinetex 5 µm C18 100 Å, LC Column 250 x 4.6 mm, Ea

Brand Name:

Kinetex

Part No:

00G-4601-E0

Phase Name:

C18

Elution Type:

Gradient

Mobile Phase:

A: Mix 3 volumes of acetonitrile R1 and 97 volumes of 3.12 g/L solution of sodium dihydrogen phosphate R previously adjusted to pH 3.8 with dilute phosphoric acid R

B: Mix 20.5 volumes of acetonitrile R1 and 79.5 volumes of 3.12 g/L solution of phosphoric acid R

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	100
2	2	100
3	37	0	100
4	62	0	100
5	62.5	100
6	66	100

Flow Rate:

1.6 mL/min

Temperature:

50°C

System:

Agilent Technologies Agilent Technologies

Detection:

LC/UV (DAD, PDA) @ 210.0000000000 nm (nanometers)

Order Items Used in This Application

00G-4601-E0

Kinetex 5 µm C18 100 Å, LC Column 250 x 4.6 mm, Ea

Qty:

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