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Applications
USP Doxepin Assay - Standard Solution on Kinetex® 2.6 µm C8 75 x 4.6 mm
26361
Separation Mode: Reversed Phase
Reversed Phase
USP
C8
HPLC
Antipruritics
Pharmaceutical/Biopharmaceutical
Pharmaceutical
Therapeutic / Clinical

Applications

USP Doxepin Assay - Standard Solution on Kinetex® 2.6 µm C8 75 x 4.6 mm
Analytes
1(E)-Doxepin
2(Z)-Doxepin
Compound Name
Doxepin
CID
667477
Molecular Formula
C19H21NO
Molecular Weight
279
No. Hydrogen Bond Acceptors
2
Details
Official Method: USP
LC Conditions (App ID: 26361)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C8
Elution Type:
Isocratic
Mobile Phase:
A: 27.6 g/L monobasic sodium phosphate, pH 2.5
B: methanol
Gradient Profile:
Step No.Time(min)%A%B
107030
Flow Rate:
1.32 mL/min
Temperature:
50°C
System:
Agilent Technologies 1290 Infinity Agilent Technologies 1290 Infinity
Detection:
LC/UV (DAD, PDA) @ 254.0000000000 nm (nanometers)
Sample Note:
Standard solution/sample solution: 0.1 mg/mL of USP Doxepin Hydrochloride RS in Mobile phase.

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