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Can I switch from non-denaturing to denaturing conditions in SEC and back again?

GENERAL

BIOZEN HPLC UHPLC SIZE EXCLUSION PHARMA

Last Updated: 4/23/2018

Commonly used denaturants in SEC included guanidine HCl, urea, and SDS. All of
these compounds can be removed from an aqueous size exclusion column, however
traces may remain in the column and/or HPLC system. Therefore, it is important to
flush thoroughly, and then test the performance of the system with a known protein
before continuing with non-denaturing conditions.